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All cell lines were grown as adherent monolayers in an essential medium supplemented with 10% FBS and 1% penicillin/streptomycin in a humid atmosphere incubator at 37 C with 5% CO 2

Arnica cream may help with any bruising.

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Our analysis clearly demonstrated that DMSO cannot be considered biologically inert but induces large alterations in microRNAs (miRNA) and epigenetic landscape, especially in the maturing cardiac model

This is especially important in tissues with high energy demands, such as the heart, skeletal muscles, and liver

10.1016/j.otsr.2010.03.010 55 MacvaninM.GluvicZ.RadovanovicJ.EssackM.GaoX.IsenovicE